Flowthrough of 239PU and 55FE during RNA extraction
| Author(s) | Manglass, Lisa M. | |
| Author(s) | Vogel, Charlotte M. | |
| Author(s) | Wintenberg, Molly | |
| Author(s) | Blenner, Mark A. | |
| Author(s) | Martinez, Nicole E. | |
| Date Accessioned | 2023-03-21T18:10:01Z | |
| Date Available | 2023-03-21T18:10:01Z | |
| Publication Date | 2023-01-31 | |
| Description | This is an author-created, un-copyedited version of an article accepted for publication/published in Journal of Radiological Protection. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.1088/1361-6498/acb15d. This article will be embargoed until 01/31/2024. | |
| Abstract | Analysis of gene expression has become an important tool in understanding low-dose effect mechanisms of ionizing radiation at the cellular level. Metal binding to nucleic acids needs to be considered when interpreting these results, as some radioactive metals, particularly actinides, may produce free radicals and cause oxidative stress damage via chemical means at rates much higher than free radical formation related to their radiological properties. Bacteria exposed in situ to low dose rates of plutonium-239 (239Pu) and iron-55 (55Fe) were previously analysed for gene expression. The work herein was motivated by an interest in more precisely identifying the distribution of radionuclides in these bacteria as well as the practical need to ensure appropriate transport and handling of the associated ribonucleic acid (RNA) extractions. RNA extractions were performed on bacteria growth media with and without bacteria cells (i.e. with and without RNA) at several different concentrations of 239Pu and 55Fe to inform the level of specificity of the extraction membrane as well as provide insight into internal (uptake) vs external (sorption) accumulation of these radionuclides in bacteria cells. Results of the study suggest that 239Pu and 55Fe detected in RNA extraction samples during long term cell studies is the result of binding to RNA prior to the time of extraction, as opposed to flow through or binding after cell lysis, and it highlights the practical importance of nucleic acid sample characterization to radiation protection more generally. | |
| Sponsor | Funding for this work was provided by the United States Defense Threat Reduction Agency under Award No. HDTRA1-17-1-0002 | |
| Citation | Manglass, L. M., Vogel, C. M., Wintenberg, M., Blenner, M. A., & Martinez, N. E. (2023). Flowthrough of 239PU and 55FE during RNA extraction. Journal of Radiological Protection, 43(1), 013502. https://doi.org/10.1088/1361-6498/acb15d | |
| ISSN | 1361-6498 | |
| URL | https://udspace.udel.edu/handle/19716/32544 | |
| Language | en_US | |
| Publisher | Journal of Radiological Protection | |
| Title | Flowthrough of 239PU and 55FE during RNA extraction | |
| Type | Article |
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