Nanoparticle Internalization Promotes the Survival of Primary Macrophages
| Author(s) | Jarai, Bader M. | |
| Author(s) | Fromen, Catherine A. | |
| Ordered Author | Bader M. Jarai, Catherine A. Fromen | en_US |
| UD Author | Catherine A. Fromen | en_US |
| UD Author | Bader M. Jarai | |
| Date Accessioned | 2022-04-07T17:25:23Z | |
| Date Available | 2022-04-07T17:25:23Z | |
| Copyright Date | Copyright © 2022 The Authors. | en_US |
| Publication Date | 2022-02-09 | |
| Description | Final published version. | en_US |
| Abstract | Macrophages, a class of tissue resident innate immune cells, are responsible forsequestering foreign objects through the process of phagocytosis, making them apromising target for immune modulation via particulate engineering. Herein, it isreported that nanoparticle (NP) dosing and cellular internalization via phago-cytosis significantly enhance survival of ex vivo cultures of primary bone marrow-derived, alveolar, and peritoneal macrophages over particle-free controls. Theenhanced survival is attributed to suppression of caspase-dependent apoptosisand is linked to phagocytosis and lysosomal signaling. Uniquely, poly(ethyleneglycol)-based NP treatment extends cell viability in the absence of macrophagepolarization and enhances expression of prosurvival B cell lymphoma-2 (Bcl-2)protein in macrophages following multiple routes of in vivo administration. Theenhanced survival phenomenon is also applicable to NPs of alternative chem-istries, indicating the potential universality of this phenomenon with relevantdrug delivery particles. Thesefindings provide a framework for extending thelifespan of primary macrophages ex vivo for drug screening, vaccine studies,and cell therapies and have implications for in vivo particulate immune-engineering applications. | en_US |
| Department | University of Delaware, Department of Chemical and Biomolecular Engineering. | en_US |
| Citation | Jarai, B.M. and Fromen, C.A. (2022), Nanoparticle Internalization Promotes the Survival of Primary Macrophages. Adv. NanoBiomed Res. 2100127. https://doi.org/10.1002/anbr.202100127 | en_US |
| DOI | doi.org/10.1002/anbr.202100127 | en_US |
| ISSN | 2699-9307 | en_US |
| URL | https://udspace.udel.edu/handle/19716/30766 | |
| Language | en_US | en_US |
| Publisher | Wiley-VCH GmbH | en_US |
| dc.rights | All articles are published under the terms of the Creative Commons Attribution License (Attribution 4.0 International) with copyright retained by the authors. | en_US |
| dc.source | Advanced NanoBiomed Research | en_US |
| dc.source.uri | https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202100127 | en_US |
| Keywords | Macrophages | en_US |
| Keywords | Nanoparticle (NP) dosing | en_US |
| Keywords | Cellular internalization | en_US |
| Keywords | Phagocytosis | en_US |
| Title | Nanoparticle Internalization Promotes the Survival of Primary Macrophages | en_US |
| Type | Article | en_US |
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