Nanoparticle Internalization Promotes the Survival of Primary Macrophages
Date
2022-02-09
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley-VCH GmbH
Abstract
Macrophages, a class of tissue resident innate immune cells, are responsible forsequestering foreign objects through the process of phagocytosis, making them apromising target for immune modulation via particulate engineering. Herein, it isreported that nanoparticle (NP) dosing and cellular internalization via phago-cytosis significantly enhance survival of ex vivo cultures of primary bone marrow-derived, alveolar, and peritoneal macrophages over particle-free controls. Theenhanced survival is attributed to suppression of caspase-dependent apoptosisand is linked to phagocytosis and lysosomal signaling. Uniquely, poly(ethyleneglycol)-based NP treatment extends cell viability in the absence of macrophagepolarization and enhances expression of prosurvival B cell lymphoma-2 (Bcl-2)protein in macrophages following multiple routes of in vivo administration. Theenhanced survival phenomenon is also applicable to NPs of alternative chem-istries, indicating the potential universality of this phenomenon with relevantdrug delivery particles. Thesefindings provide a framework for extending thelifespan of primary macrophages ex vivo for drug screening, vaccine studies,and cell therapies and have implications for in vivo particulate immune-engineering applications.
Description
Final published version.
Keywords
Macrophages, Nanoparticle (NP) dosing, Cellular internalization, Phagocytosis
Citation
Jarai, B.M. and Fromen, C.A. (2022), Nanoparticle Internalization Promotes the Survival of Primary Macrophages. Adv. NanoBiomed Res. 2100127. https://doi.org/10.1002/anbr.202100127