Browsing by Author "Raciti, David"
Now showing 1 - 1 of 1
Results Per Page
Sort Options
Item Activity of (-) Astrogorgiadiol(University of Delaware, 2012-05) Raciti, David(-) Astrogorgiadiol, a naturally occurring analog of calcitriol (1,25-dihydroxyvitamin D3) isolated from the Japanese marine sponge Astrogorgia, has demonstrated unique biological functions. In an effort to study these functions, synthetic routes for obtaining (-) astrogorgiadiol were constructed in 2001 by Dr. Taber and Dr. Malcolm. This thesis reports the several efforts made to increase the cost-effectiveness and productivity of synthetic routes leading to (-) astrogorgiadiol. While pursuing more cost-effective and productive means to synthesize (-) astrogorgiadiol, several milligrams of the compound were obtained and used for biological study. The question that was proposed and investigated was, “What effects does (-) astrogorgiadiol elicit on cancer cells?” To begin gathering data to answer this question, several in-vitro assays were conducted on various cell lines. First, the LD50/ED50 for (-) astrogorgiadiol was determined utilizing two bone cancer cell lines. Then utilizing the LD50/ED50 concentration, cells were exposed to (-) astrogorgiadiol and expression studies were pursued on Osteopontin (OPN), a glycoprotein known for its over-expression in various cancers.