Genome divergence and reproductive incompatibility among populations of Ganaspis near brasiliensis
| Author(s) | Hopper, Keith R. | |
| Author(s) | Wang, Xingeng | |
| Author(s) | Kenis, Marc | |
| Author(s) | Seehausen, M. Lukas | |
| Author(s) | Abram, Paul K. | |
| Author(s) | Daane, Kent M. | |
| Author(s) | Buffington, Matthew L. | |
| Author(s) | Hoelmer, Kim A. | |
| Author(s) | Kingham, Brewster F. | |
| Author(s) | Shevchenko, Olga | |
| Author(s) | Bernberg, Erin | |
| Date Accessioned | 2024-05-16T17:34:04Z | |
| Date Available | 2024-05-16T17:34:04Z | |
| Publication Date | 2024-05-08 | |
| Description | This article was originally published in Genes | Genomes | Genetics. The version of record is available at: https://doi.org/10.1093/g3journal/jkae090. Published by Oxford University Press on behalf of The Genetics Society of America 2024. This work is written by (a) US Government employee(s) and is in the public domain in the US. | |
| Abstract | During the last decade, the spotted wing drosophila, Drosophila suzukii, has spread from eastern Asia to the Americas, Europe, and Africa. This fly attacks many species of cultivated and wild fruits with soft, thin skins, where its serrated ovipositor allows it to lay eggs in undamaged fruit. Parasitoids from the native range of D. suzukii may provide sustainable management of this polyphagous pest. Among these parasitoids, host-specificity testing has revealed a lineage of Ganaspis near brasiliensis, referred to in this paper as G1, that appears to be a cryptic species more host-specific to D. suzukii than other parasitoids. Differentiation among cryptic species is critical for introduction and subsequent evaluation of their impact on D. suzukii. Here, we present results on divergence in genomic sequences and architecture and reproductive isolation between lineages of Ganaspis near brasiliensis that appear to be cryptic species. We studied five populations, two from China, two from Japan, and one from Canada, identified as the G1 vs G3 lineages based on differences in cytochrome oxidase l sequences. We assembled and annotated the genomes of these populations and analyzed divergences in sequence and genome architecture between them. We also report results from crosses to test reproductive compatibility between the G3 lineage from China and the G1 lineage from Japan. The combined results on sequence divergence, differences in genome architectures, ortholog divergence, reproductive incompatibility, differences in host ranges and microhabitat preferences, and differences in morphology show that these lineages are different species. Thus, the decision to evaluate the lineages separately and only import and introduce the more host-specific lineage to North America and Europe was appropriate. | |
| Sponsor | This research was supported by the United States Department of Agriculture, Agricultural Research Service, Project Numbers 8010-22000-032-00D and 8010-22000-033-00D, and the USDA Specialty Crop Research Initiative Award No. 2020-51181-32140. Support from the University of Delaware Bioinformatics Core Facility, including use of the BIOMIX computational cluster, was made possible by the Delaware IDeA Network of Biomedical Research Excellence (National Institutes of Health grant P20 GM103446). | |
| Citation | Keith R Hopper, Xingeng Wang, Marc Kenis, M Lukas Seehausen, Paul K Abram, Kent M Daane, Matthew L Buffington, Kim A Hoelmer, Brewster F Kingham, Olga Shevchenko, Erin Bernberg, Genome divergence and reproductive incompatibility among populations of Ganaspis near brasiliensis, G3 Genes|Genomes|Genetics, 2024;, jkae090, https://doi.org/10.1093/g3journal/jkae090 | |
| ISSN | 2160-1836 | |
| URL | https://udspace.udel.edu/handle/19716/34411 | |
| Language | en_US | |
| Publisher | Genes | Genomes | Genetics | |
| Keywords | genome assembly | |
| Keywords | genome annotation | |
| Keywords | reproductive incompatibility | |
| Keywords | biological control | |
| Keywords | parasitoid | |
| Keywords | Hymenoptera | |
| Title | Genome divergence and reproductive incompatibility among populations of Ganaspis near brasiliensis | |
| Type | Article |
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