A short-term, randomized, controlled, feasibility study of the effects of different vegetables on the gut microbiota and microRNA expression in infants

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Author(s)Ferro, Lynn E.
Author(s)Bittinger, Kyle
Author(s)Trudo, Sabrina P.
Author(s)Beane, Kaleigh E.
Author(s)Polson, Shawn W.
Author(s)Kim, Jae Kyeom
Author(s)Trabulsi, Jillian C.
Date Accessioned2024-04-25T15:32:50Z
Date Available2024-04-25T15:32:50Z
Publication Date2024-03-01
DescriptionThis article was originally published in Frontiers in Microbiomes. The version of record is available at: https://doi.org/10.3389/frmbi.2024.1342464. © 2024 Ferro, Bittinger, Trudo, Beane, Polson, Kim and Trabulsi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
AbstractThe complementary diet influences the gastrointestinal (gut) microbiota composition and, in turn, host health and, potentially, microRNA (miRNA) expression. This study aimed to assess the feasibility of altering the gut microbial communities with short-term food introduction and to determine the effects of different vegetables on the gut microbiota and miRNA expression in infants. A total of 11 infants were randomized to one of the following intervention arms: control, broccoli, or carrot. The control group maintained the milk diet only, while the other groups consumed either a broccoli puree or a carrot puree on days 1–3 along with their milk diet (human milk or infant formula). Genomic DNA and total RNA were extracted from fecal samples to determine the microbiota composition and miRNA expression. Short-term feeding of both broccoli and carrots resulted in changes in the microbiota and miRNA expression. Compared to the control, a trend toward a decrease in Shannon index was observed in the carrot group on days 2 and 4. The carrot and broccoli groups differed by weighted UniFrac. Streptococcus was increased on day 4 in the carrot group compared to the control. The expression of two miRNAs (i.e., miR-217 and miR-590-5p) trended towards decrease in both the broccoli and carrot groups compared to the control, whereas increases in eight and two different miRNAs were observed in the carrot and broccoli groups, respectively. Vegetable interventions differentially impacted the gut microbiota and miRNA expression, which may be a mechanism by which total vegetable intake and variety are associated with reduced disease risk.
SponsorThe author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported by the University of Delaware’s Predoctoral Summer Fellowship and Doctoral Fellowship for Excellence, two Institutional Development Awards (IDeA) from the National Institute of Health’s National Institute of General Medical Sciences and Delaware INBRE (NIH P20 GM103446), the State of Delaware, the Delaware Biotechnology Institute, and the University of Arkansas Chancellor’s Grant.
CitationFerro LE, Bittinger K, Trudo SP, Beane KE, Polson SW, Kim JK and Trabulsi JC (2024) A short-term, randomized, controlled, feasibility study of the effects of different vegetables on the gut microbiota and microRNA expression in infants. Front. Microbiomes 3:1342464. doi: 10.3389/frmbi.2024.1342464
ISSN2813-4338
URLhttps://udspace.udel.edu/handle/19716/34306
Languageen_US
PublisherFrontiers in Microbiomes
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
Keywordsapiaceae
Keywordsbroccoli
Keywordscarrot
KeywordsCruciferae
Keywordsinfant feeding
Keywordsmicrobiome
TitleA short-term, randomized, controlled, feasibility study of the effects of different vegetables on the gut microbiota and microRNA expression in infants
TypeArticle
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