Facilitating viral vector movement enhances heterologous protein production in an established plant system

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Author(s)Wang, Xu
Author(s)Prokhnevsky, Alexei I.
Author(s)Skarjinskaia, Marina
Author(s)Razzak, Md Abdur
Author(s)Streatfield, Stephen J.
Author(s)Lee, Jung-Youn
Date Accessioned2023-02-16T20:37:20Z
Date Available2023-02-16T20:37:20Z
Publication Date2023-01-04
DescriptionThis article was originally published in Plant Biotechnology Journal. The version of record is available at: https://doi.org/10.1111/pbi.13977
AbstractMolecular farming technology using transiently transformed Nicotiana plants offers an economical approach to the pharmaceutical industry to produce an array of protein targets including vaccine antigens and therapeutics. It can serve as a desirable alternative approach for those proteins that are challenging or too costly to produce in large quantities using other heterologous protein expression systems. However, since cost metrics are such a critical factor in selecting a production host, any system-wide modifications that can increase recombinant protein yields are key to further improving the platform and making it applicable for a wider range of target molecules. Here, we report on the development of a new approach to improve target accumulation in an established plant-based expression system that utilizes viral-based vectors to mediate transient expression in Nicotiana benthamiana. We show that by engineering the host plant to support viral vectors to spread more effectively between host cells through plasmodesmata, protein target accumulation can be increased by up to approximately 60%.
SponsorWe thank J. Caplan (Univ. Delaware) for sharing the GV2260 agrobacterial cell carrying the TMV-GFP virus. This research was supported primarily by Fraunhofer USA under a task agreement awarded to J.-Y. Lee and A.I. Prokhnevsky made under a partnership agreement between Fraunhofer USA and the University of Delaware and partially by the National Science Foundation (awarded to J.-Y. Lee: NSF MCB-1820103 and IOS-2054685).
CitationWang, X., Prokhnevsky, A.I., Skarjinskaia, M., Razzak, M.A., Streatfield, S.J. and Lee, J.-Y. (2023), Facilitating viral vector movement enhances heterologous protein production in an established plant system. Plant Biotechnol J. https://doi.org/10.1111/pbi.13977
ISSN1467-7652
URLhttps://udspace.udel.edu/handle/19716/32310
Languageen_US
PublisherPlant Biotechnology Journal
Keywordsmolecular farming
Keywordsplantbased pharmaceutical
Keywordstobacco mosaic virus
Keywordsviral expression vector
Keywordsplasmodesmata
Keywordscell-to-cell movement
TitleFacilitating viral vector movement enhances heterologous protein production in an established plant system
TypeArticle
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