microRNA-124 regulates Notch and NeuroD1 to mediate transition states of neuronal development

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Author(s)Konrad, Kalin D.
Author(s)Song, Jia L.
Date Accessioned2023-01-06T19:26:13Z
Date Available2023-01-06T19:26:13Z
Publication Date2022-11-23
DescriptionThis is the peer reviewed version of the following article: Konrad, K. D., & Song, J. L. (2022). microRNA-124 regulates Notch and NeuroD1 to mediate transition states of neuronal development. Developmental Neurobiology, 00, 1– 25. https://doi.org/10.1002/dneu.22902, which has been published in final form at https://doi.org/10.1002/dneu.22902. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. This article will be embargoed until 11/23/2023.
AbstractMicroRNAs regulate gene expression by destabilizing target mRNA and/or inhibiting translation in animal cells. The ability to mechanistically dissect miR-124′s function during specification, differentiation, and maturation of neurons during development within a single system has not been accomplished. Using the sea urchin embryo, we take advantage of the manipulability of the embryo and its well-documented gene regulatory networks (GRNs). We incorporated NeuroD1 as part of the sea urchin neuronal GRN and determined that miR-124 inhibition resulted in aberrant gut contractions, swimming velocity, and neuronal development. Inhibition of miR-124 resulted in an increased number of cells expressing transcription factors (TFs) associated with progenitor neurons and a concurrent decrease of mature and functional neurons. Results revealed that in the early blastula/gastrula stages, miR-124 regulates undefined factors during neuronal specification and differentiation. In the late gastrula/larval stages, miR-124 regulates Notch and NeuroD1 during the transition between neuronal differentiation and maturation. Overall, we have improved the neuronal GRN and identified miR-124 to play a prolific role in regulating various transitions of neuronal development.
SponsorThis work is funded by National Science Foundation (IOS 1553338 and MCB 2103453 to J.L.S.), NIH P20GM103653, NIH NIGMS P20GM103446, and Sigma Xi Grants-in-Aid of Research (G2018031596227966 to K.D.K.).
CitationKonrad, K. D., & Song, J. L. (2022). microRNA-124 regulates Notch and NeuroD1 to mediate transition states of neuronal development. Developmental Neurobiology, 00, 1– 25. https://doi.org/10.1002/dneu.22902
ISSN1932-846X
URLhttps://udspace.udel.edu/handle/19716/31996
Languageen_US
PublisherDevelopmental Neurobiology
Keywordssea urchin
Keywordspost-transcriptional regulation
Keywordsgene regulatory network
Keywordsneurogenesis
Keywordssignaling pathways
TitlemicroRNA-124 regulates Notch and NeuroD1 to mediate transition states of neuronal development
TypeArticle
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