Modeling Cataract Surgery in Mice
| Author(s) | O'Neill, Leah M. | |
| Author(s) | Wang, Yan | |
| Author(s) | Duncan, Melinda K. | |
| Date Accessioned | 2024-03-08T18:28:39Z | |
| Date Available | 2024-03-08T18:28:39Z | |
| Publication Date | 2023-12-01 | |
| Description | This article was originally published in Journal of Visualized Experiments. The version of record is available at: https://doi.org/10.3791/66050. Copyright © 2023 JoVE Journal of Visualized Experiments. This article will be embargoed until 12/01/2025. | |
| Abstract | Cataract surgery (CS) is an effective treatment for cataracts, a major cause of visual disability worldwide. However, CS leads to ocular inflammation, and in the long term, it can result in posterior capsular opacification (PCO) and/or lens dislocation driven by the post-surgical overgrowth of lens epithelial cells (LECs) and their conversion to myofibroblasts and/or aberrant fiber cells. However, the molecular mechanisms by which CS results in inflammation and PCO are still obscure because most in vitro models do not recapitulate the wound healing response of LECs seen in vivo, while traditional animal models of cataract surgery, such as rabbits, do not allow the genetic manipulation of gene expression to test mechanisms. Recently, our laboratory and others have successfully used genetically modified mice to study the molecular mechanisms that drive the induction of proinflammatory signaling and LEC epithelial to mesenchymal transition, leading to new insight into PCO pathogenesis. Here, we report the established protocol for modeling cataract surgery in mice, which allows for robust transcriptional profiling of the response of LECs to lens fiber cell removal via RNAseq, the evaluation of protein expression by semi-quantitative immunofluorescence, and the use of modern mouse genetics tools to test the function of genes that are hypothesized to participate in the pathogenesis of acute sequelae like inflammation as well as the later conversion of LECs to myofibroblasts and/or aberrant lens fiber cells. | |
| Sponsor | This work was supported by the National Eye Institute (EY015279 and EY028597) and Delaware INBRE (P20 GM103446). Disclosures The Duncan lab has received financial support from Pliantx to test anti-PCO therapies using this surgery model. | |
| Citation | O'Neill, L. M., Wang, Y., Duncan, M. K. Modeling Cataract Surgery in Mice. J. Vis. Exp. (202), e66050, doi:10.3791/66050 (2023). | |
| ISSN | 1940-087X | |
| URL | https://udspace.udel.edu/handle/19716/34154 | |
| Language | en_US | |
| Publisher | Journal of Visualized Experiments | |
| Title | Modeling Cataract Surgery in Mice | |
| Type | Article |
Files
Original bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- Modeling Cataract Surgery in Mice.pdf
- Size:
- 1.87 MB
- Format:
- Adobe Portable Document Format
- Description:
- Main article
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 2.22 KB
- Format:
- Item-specific license agreed upon to submission
- Description: