RNA localization to the mitotic spindle is essential for early development and is regulated by kinesin-1 and dynein
Date
2023-03-06
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Cell Science
Abstract
Mitosis is a fundamental and highly regulated process that acts to faithfully segregate chromosomes into two identical daughter cells. Localization of gene transcripts involved in mitosis to the mitotic spindle might be an evolutionarily conserved mechanism to ensure that mitosis occurs in a timely manner. We identified many RNA transcripts that encode proteins involved in mitosis localized at the mitotic spindles in dividing sea urchin embryos and mammalian cells. Disruption of microtubule polymerization, kinesin-1 or dynein results in lack of spindle localization of these transcripts in the sea urchin embryo. Furthermore, results indicate that the cytoplasmic polyadenylation element (CPE) within the 3′UTR of the Aurora B transcript, a recognition sequence for CPEB, is essential for RNA localization to the mitotic spindle in the sea urchin embryo. Blocking this sequence results in arrested development during early cleavage stages, suggesting that RNA localization to the mitotic spindle might be a regulatory mechanism of cell division that is important for early development.
Description
This article was originally published in Journal of Cell Science. The version of record is available at: https://doi.org/10.1242/jcs.260528. This article will be embargoed until 3/6/2024.
Keywords
Mitosis, RNA localization, Kinesin-1, Dynein, Embryonic development
Citation
Remsburg, Carolyn M., Kalin D. Konrad, and Jia L. Song. “RNA Localization to the Mitotic Spindle Is Essential for Early Development and Is Regulated by Kinesin-1 and Dynein.” Journal of Cell Science 136, no. 5 (March 2023): jcs260528. https://doi.org/10.1242/jcs.260528.