CHARACTERIZATION OF NANOPARTICLE LOADING IN AEROSOLS FOR PULMONARY NUCLEIC ACID DELIVERY
| Author(s) | Gill, Nicole | |
| Date Accessioned | 2024-06-12T17:09:51Z | |
| Date Available | 2024-06-12T17:09:51Z | |
| Publication Date | 2024-05 | |
| Abstract | Approximately 544.9 million people globally are diagnosed with chronic respiratory diseases, which has increased 39.8% since 1990. 1 Aerosolized drugs that can yield controlled effects on the pulmonary immune system may afford new opportunities to treat such diseases. In this research, we investigate the role of nanoparticle formulations as respirable drug delivery carriers. We first investigate the physical effects of nanoparticle loading into nebulized aerosols to create respirable aerosols with tunable sizes. Formulations of different concentrations of nanoparticles were aerosolized with an Aeroneb vibrating mesh nebulizer and characterized by laser diffraction. We find that at a critical nanoparticle concentration, the aerosol volumetric median diameter increased upwards of ~150%. Methods of nucleic acid delivery for pulmonary applications were also explored. Polyplexes were dosed to lung epithelial cells using both liquid and aerosol methods, in which cell transfection was induced successfully using both delivery methods. The results presented in this work have the potential to have significant impacts on particulate aerosol delivery systems, as well as nucleic acid delivery applications. Future work includes combining these physical and cellular effects to improve the transport and delivery of bio-active nanoparticles for immune and gene delivery applications. | |
| Advisor | enter | |
| Program | enter | |
| URL | https://udspace.udel.edu/handle/19716/34485 | |
| Language | en_US | |
| Publisher | University of Delaware | |
| Title | CHARACTERIZATION OF NANOPARTICLE LOADING IN AEROSOLS FOR PULMONARY NUCLEIC ACID DELIVERY | |
| Type | Thesis |