Cultivation-dependent and cultivation-independent characterization of hydrocarbon-degrading bacteria in Guaymas Basin sediments.
| Author(s) | Gutierrez, Tony | |
| Author(s) | Biddle, Jennifer F. | |
| Author(s) | Teske, Andreas | |
| Author(s) | Aitken, Michael D. | |
| Ordered Author | Tony Gutierrez, Jennifer F. Biddle, Andreas Teske and Michael D. Aitken | |
| UD Author | Biddle, Jennifer F. | en_US |
| Date Accessioned | 2015-12-16T14:55:44Z | |
| Date Available | 2015-12-16T14:55:44Z | |
| Copyright Date | Copyright © 2015 Gutierrez, Biddle, Teske and Aitken. | en_US |
| Publication Date | 2015-07-07 | |
| Description | Publisher's PDF. | en_US |
| Abstract | Marine hydrocarbon-degrading bacteria perform a fundamental role in the biodegradation of crude oil and its petrochemical derivatives in coastal and open ocean environments. However, there is a paucity of knowledge on the diversity and function of these organisms in deep-sea sediment. Here we used stable-isotope probing (SIP), a valuable tool to link the phylogeny and function of targeted microbial groups, to investigate polycyclic aromatic hydrocarbon (PAH)-degrading bacteria under aerobic conditions in sediments from Guaymas Basin with uniformly labeled [(13)C]-phenanthrene (PHE). The dominant sequences in clone libraries constructed from (13)C-enriched bacterial DNA (from PHE enrichments) were identified to belong to the genus Cycloclasticus. We used quantitative PCR primers targeting the 16S rRNA gene of the SIP-identified Cycloclasticus to determine their abundance in sediment incubations amended with unlabeled PHE and showed substantial increases in gene abundance during the experiments. We also isolated a strain, BG-2, representing the SIP-identified Cycloclasticus sequence (99.9% 16S rRNA gene sequence identity), and used this strain to provide direct evidence of PHE degradation and mineralization. In addition, we isolated Halomonas, Thalassospira, and Lutibacterium sp. with demonstrable PHE-degrading capacity from Guaymas Basin sediment. This study demonstrates the value of coupling SIP with cultivation methods to identify and expand on the known diversity of PAH-degrading bacteria in the deep-sea. | en_US |
| Department | University of Delaware. College of Earth, Ocean, and Environment. | en_US |
| Citation | Gutierrez T,Biddle JF,Teske A and Aitken MD (2015) Cultivation-dependent and cultivation-independent characterization of hydrocarbon-degrading bacteria in Guaymas Basin sediments. Front. Microbiol.6:695. doi:10.3389/fmicb.2015.00695 | en_US |
| DOI | doi:10.3389/fmicb.2015.00695 | en_US |
| ISSN | 1664-302X | en_US |
| URL | http://udspace.udel.edu/handle/19716/17319 | |
| Language | en_US | en_US |
| Publisher | Frontiers Media S.A. | en_US |
| dc.rights | CC-BY 4.0. The authors hold the copyright. This document is protected by copyright and was first published by Frontier. All rights reserved. It is reproduced with permission. | en_US |
| dc.source | Frontiers in Microbiology | en_US |
| dc.source.uri | http://journal.frontiersin.org/journal/microbiology | en_US |
| Title | Cultivation-dependent and cultivation-independent characterization of hydrocarbon-degrading bacteria in Guaymas Basin sediments. | en_US |
| Type | Article | en_US |
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