Effect of Homo-Fermentative Lactic Acid Bacteria Inoculants on Fermentation Characteristics and Bacterial and Fungal Communities in Alfalfa Silage

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Author(s)Li, Yanbing
Author(s)da Silva, E. B.
Author(s)Li, Jingchun
Author(s)Kung, L. Jr.
Date Accessioned2023-01-10T16:47:53Z
Date Available2023-01-10T16:47:53Z
Publication Date2022-11-10
DescriptionThis article was originally published in Fermentation. The version of record is available at: https://doi.org/10.3390/fermentation8110621
AbstractWe evaluated the effects of a homo-fermentative lactic acid bacteria (homo-LAB) inoculant on the fermentation and microbial communities of alfalfa ensiled at two dry matter (DM) contents of 38 and 46% DM. At both DMs, alfalfa was treated or not with an inoculant containing Pediococcus acidilactici, Enterococcus faecium and Lactobacillus plantarum at a targeted application rate of 165,000 cfu/g of fresh weight and stored for 3, 30 and 60 days. Treatment with the inoculant resulted in a lower drop in pH and, in general, higher lactic acid and lower acetic acid when applied to medium DM silage. For the four most abundant microbial genera, increased abundances of Bacteroides and Lactobacillus (p < 0.05), as well as decreased abundances of Muribaculaceae were observed in high DM and inoculated silages. The abundance of Prevotellaceae-UCG-001 was lower in medium DM control silages than in high DM control silages. Inoculation and DM affected abundances of Vishniacozyma (p < 0.05). Increased abundances of Vishniacozyma, as well as decreased abundances of Leucosporidium were observed in medium DM-inoculated silages. Changes in the relative abundance (RA) of the main populations of bacteria and yeasts did explain the fermentation and nutrition differences among treatments.
SponsorThis study was supported by the Joint Fund for National Natural Science Foundation of China (Grant No. 31402136). This research was partially supported by Purina Animal Nutrition LLC, Shoreview, MN. The authors thank the farm staff of the University of Delaware farm for assistance with forage harvesting and Michelle Der Bedrosian for silo preparation.
CitationLi, Yanbing, E. B. da Silva, Jingchun Li, and L. Kung Jr. 2022. "Effect of Homo-Fermentative Lactic Acid Bacteria Inoculants on Fermentation Characteristics and Bacterial and Fungal Communities in Alfalfa Silage" Fermentation 8, no. 11: 621. https://doi.org/10.3390/fermentation8110621
ISSN2311-5637
URLhttps://udspace.udel.edu/handle/19716/32041
Languageen_US
PublisherFermentation
Keywordsmicrobial inoculants
Keywordsalfalfa silage
Keywordsdry matter
Keywordsmicrobial community
Keywordsrelative abundance
TitleEffect of Homo-Fermentative Lactic Acid Bacteria Inoculants on Fermentation Characteristics and Bacterial and Fungal Communities in Alfalfa Silage
TypeArticle
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