Surfactin and Spo0A-Dependent Antagonism by Bacillus subtilis Strain UD1022 against Medicago sativa Phytopathogens

Simple item page
Author(s)Rosier, Amanda
Author(s)Pomerleau, Maude
Author(s)Beauregard, Pascale B.
Author(s)Samac, Deborah A.
Author(s)Bais, Harsh P.
Date Accessioned2023-06-15T15:28:27Z
Date Available2023-06-15T15:28:27Z
Publication Date2023-02-23
DescriptionThis article was originally published in Plants. The version of record is available at: https://doi.org/10.3390/plants12051007. © 2023 by the authors. Licensee MDPI, Basel, Switzerland.
AbstractPlant growth-promoting rhizobacteria (PGPR) such as the root colonizers Bacillus spp. may be ideal alternatives to chemical crop treatments. This work sought to extend the application of the broadly active PGPR UD1022 to Medicago sativa (alfalfa). Alfalfa is susceptible to many phytopathogens resulting in losses of crop yield and nutrient value. UD1022 was cocultured with four alfalfa pathogen strains to test antagonism. We found UD1022 to be directly antagonistic toward Collectotrichum trifolii, Ascochyta medicaginicola (formerly Phoma medicaginis), and Phytophthora medicaginis, and not toward Fusarium oxysporum f. sp. medicaginis. Using mutant UD1022 strains lacking genes in the nonribosomal peptide (NRP) and biofilm pathways, we tested antagonism against A. medicaginicola StC 306-5 and P. medicaginis A2A1. The NRP surfactin may have a role in the antagonism toward the ascomycete StC 306-5. Antagonism toward A2A1 may be influenced by B. subtilis biofilm pathway components. The B. subtilis central regulator of both surfactin and biofilm pathways Spo0A was required for the antagonism of both phytopathogens. The results of this study indicate that the PGPR UD1022 would be a good candidate for further investigations into its antagonistic activities against C. trifolii, A. medicaginicola, and P. medicaginis in plant and field studies.
SponsorThis research was supported by a Multistate Hatch grant from the USDA and USDA SARE.
CitationRosier, Amanda, Maude Pomerleau, Pascale B. Beauregard, Deborah A. Samac, and Harsh P. Bais. 2023. "Surfactin and Spo0A-Dependent Antagonism by Bacillus subtilis Strain UD1022 against Medicago sativa Phytopathogens" Plants 12, no. 5: 1007. https://doi.org/10.3390/plants12051007
ISSN2223-7747
URLhttps://udspace.udel.edu/handle/19716/32931
Languageen_US
PublisherPlants
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
KeywordsPGPR
Keywordsalfalfa
Keywordsantagonism
KeywordsBacillus subtilis
KeywordsPhytophthora medicaginis
KeywordsAscochyta medicaginicola
KeywordsPhoma medicaginis
Keywordssurfactin
Keywordsbiofilm
TitleSurfactin and Spo0A-Dependent Antagonism by Bacillus subtilis Strain UD1022 against Medicago sativa Phytopathogens
TypeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Surfactin and Spo0A Dependent Antagonism by Bacillus subtilis Strain UD1022 against Medicago sativa Phytopathogens.pdf
Size:
1.67 MB
Format:
Adobe Portable Document Format
Description:
Main article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.22 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Open Access Publications