THE EFFECT OF HYPOXIA ON THE EXPRESSION OF ISOCITRATE LYASE IN TETRAHYMENA THERMOPHILA
| Author(s) | Rainey, Jessica | |
| Date Accessioned | 2020-09-21T16:35:48Z | |
| Date Available | 2020-09-21T16:35:48Z | |
| Publication Date | 2020-05 | |
| Abstract | Tetrahymena thermophila are ciliates that live in freshwater environments. Little is known about their reaction to hypoxic stress. Hypoxia is thought to induce the glyoxylate cycle, a variant of the tricarboxylic acid cycle. Older studies in Tetrahymena pyriformis tested this by growing cells in containers with a very small surface area to simulate hypoxia and containers with a larger surface area be a normoxia control. These studies looked at the biochemical activities of two critical enzymes of the glyoxylate cycle, isocitrate lyase and malate synthase. The current study is interested in determining if there is a change in the gene expression or activity of isocitrate lyase, a glyoxylate enzyme, in T. thermophila in carefully defined hypoxic conditions. This was explored through the use of qPCR and biochemical ICL assays. The qPCR trials found a 3.16-fold increase in the expression of ICL, although the data were inconsistent and not significant. The ICL activity experiments were inconclusive but did not seem to indicate a change in ICL activity. In conclusion, hypoxia may increase ICL expression or activity, but further work is needed. | en_US |
| Advisor | Gary Laverty, Ph.D. | |
| Program | Biological Sciences | |
| URL | https://udspace.udel.edu/handle/19716/27731 | |
| Publisher | University of Delaware | en_US |
| Keywords | biological science, hypoxia, isocitrate lyase, tetrahymena thermophila | en_US |
| Title | THE EFFECT OF HYPOXIA ON THE EXPRESSION OF ISOCITRATE LYASE IN TETRAHYMENA THERMOPHILA | en_US |
| Type | Thesis | en_US |