Browsing by Author "Swenson, Rolf"
Now showing 1 - 1 of 1
Results Per Page
Sort Options
Item Exploring Sulfur Sites in Proteins via Triple-Resonance 1H-Detected 77Se NMR(Journal of the American Chemical Society, 2023-11-15) Koscielniak, Janusz; Li, Jess; Sail, Deepak; Swenson, Rolf; Anklin, Clemens; Rozovsky, Sharon; Byrd, R. AndrewNMR spectroscopy has been applied to virtually all sites within proteins and biomolecules; however, the observation of sulfur sites remains very challenging. Recent studies have examined 77Se as a replacement for sulfur and applied 77Se NMR in both the solution and solid states. As a spin-1/2 nuclide, 77Se is attractive as a probe of sulfur sites, and it has a very large chemical shift range (due to a large chemical shift anisotropy), which makes it potentially very sensitive to structural and/or binding interactions as well as dynamics. Despite being a spin-1/2 nuclide, there have been rather limited studies of 77Se, and the ability to use 1H-indirect detection has been sparse. Some examples exist, but in the absence of a directly bonded, nonexchangeable 1H, these have been largely limited to smaller molecules. We develop and illustrate approaches using double-labeling of 13C and 77Se in proteins that enable more sensitive triple-resonance schemes via multistep coherence transfers and 1H-detection. These methods require specialized hardware and decoupling schemes, which we developed and will be discussed.