Glucose detection with surface plasmon resonance spectroscopy and molecularly imprinted hydrogel coated sensors
| Author(s) | Wang, Jing | |
| Date Accessioned | 2012-11-12T17:06:27Z | |
| Date Available | 2012-11-12T17:06:27Z | |
| Publication Date | 2011 | |
| Abstract | Molecularly imprinted hydrogel membranes were developed and evaluated for detection of small analytes via surface plasmon resonance spectroscopy. Imprinting of glucose phosphate barium salt into a poly(allylamine hydrochloride) network covalently bound to gold surfaces yielded a selective sensor for glucose. Optimization of relative amounts of chemicals used for preparation of the hydrogel was performed to obtain highest sensitivity. Addition of gold nanoparticles into the hydrogel matrix significantly amplified its response and sensitivity due to the impact of gold nanoparticles on the refractive index of sensing layer. The detection limit of glucose in deionized water was calculated to be 0.02 mg/mL, well within the working concentration range suitable for glucose monitoring in diabetic individuals at physiological levels. Evaluation of its selectivity showed that the sensor displayed preferential recognition to glucose compared to structurally related sugars in addition to being unaffected by phosphate as well as compounds containing amine groups, such as creatinine. The developed sensor was finally exposed to human urine spiked with glucose illustrating its ability to selectively re-bind the analyte in complex matrices. | en_US |
| Advisor | Booksh, Karl | |
| Degree | M.S. | |
| Department | University of Delaware, Department of Chemistry and Biochemistry | |
| URL | http://udspace.udel.edu/handle/19716/11709 | |
| Publisher | University of Delaware | en_US |
| dc.subject.lcsh | Glucose. | |
| dc.subject.lcsh | Surface plasmon resonance. | |
| dc.subject.lcsh | Spectrum analysis. | |
| dc.subject.lcsh | Chemical detectors. | |
| dc.subject.lcsh | Colloids. | |
| dc.subject.lcsh | Imprinted polymers. | |
| Title | Glucose detection with surface plasmon resonance spectroscopy and molecularly imprinted hydrogel coated sensors | en_US |
| Type | Thesis | en_US |