Distinct and Cooperative Activities of HESO1 and URT1 Nucleotidyl Transferases in MicroRNA Turnover in Arabidopsis
| Author(s) | Tu, Bin | |
| Author(s) | Liu, Li | |
| Author(s) | Xu, Chi | |
| Author(s) | Zhai, Jixian | |
| Author(s) | Li, Shengben | |
| Author(s) | Lopez, Miguel A. | |
| Author(s) | Zhao, Yuanyuan | |
| Author(s) | Yu, Yu | |
| Author(s) | Ramachandran, Vanitharani | |
| Author(s) | Ren, Guodong | |
| Author(s) | Yu, Bin | |
| Author(s) | Li, Shigui | |
| Author(s) | Meyers, Blake C. | |
| Author(s) | Mo, Beixin | |
| Author(s) | Chen, Xuemei | |
| Ordered Author | Bin Tu1, Li Liu, Chi Xu, Jixian Zhai, Shengben Li, Miguel A. Lopez, Yuanyuan Zhao, Yu Yu, Vanitharani Ramachandran, Guodong Ren, Bin Yu, Shigui Li, Blake C. Meyers, Beixin Mo, Xuemei Chen | |
| UD Author | Zhai, Jixian | en_US |
| UD Author | Meyers, Blake C. | |
| Date Accessioned | 2016-03-29T19:52:26Z | |
| Date Available | 2016-03-29T19:52:26Z | |
| Copyright Date | Copyright © 2015 Tu et al. | en_US |
| Publication Date | 2015-04-30 | |
| Description | Publisher's PDF. | en_US |
| Abstract | 3’ uridylation is increasingly recognized as a conserved RNA modification process associated with RNA turnover in eukaryotes. 2’-O-methylation on the 3’ terminal ribose protects micro(mi)RNAs from 3’ truncation and 3’ uridylation in Arabidopsis. Previously, we identified HESO1 as the nucleotidyl transferase that uridylates most unmethylated miRNAs in vivo, but substantial 3’ tailing of miRNAs still remains in heso1 loss-of-function mutants. In this study, we found that among nine other potential nucleotidyl transferases, UTP:RNA URIDYLYLTRANSFERASE 1 (URT1) is the single most predominant nucleotidyl transferase that tails miRNAs. URT1 and HESO1 prefer substrates with different 3’ end nucleotides in vitro and act cooperatively to tail different forms of the same miRNAs in vivo. Moreover, both HESO1 and URT1 exhibit nucleotidyl transferase activity on AGO1-bound miRNAs. Although these enzymes are able to add long tails to AGO1-bound miRNAs, the tailed miRNAs remain associated with AGO1. Moreover, tailing of AGO1-bound miRNA165/6 drastically reduces the slicing activity of AGO1-miR165/6, suggesting that tailing reduces miRNA activity. However, monouridylation of miR171a by URT1 endows the miRNA the ability to trigger the biogenesis of secondary siRNAs. Therefore, 3’ tailing could affect the activities of miRNAs in addition to leading to miRNA degradation. | en_US |
| Department | University of Delaware. Department of Plant and Soil Sciences. | en_US |
| Department | University of Delaware. Delaware Biotechnology Institute. | en_US |
| Citation | Tu B, Liu L, Xu C, Zhai J, Li S, Lopez MA, et al. (2015) Distinct and Cooperative Activities of HESO1 and URT1 Nucleotidyl Transferases in MicroRNA Turnover in Arabidopsis. PLoS Genet 11 (4): e1005119. doi:10.1371/journal.pgen.1005119 | en_US |
| DOI | doi:10.1371/journal.pgen.1005119 | en_US |
| ISSN | 1553-7390 | en_US |
| URL | http://udspace.udel.edu/handle/19716/17574 | |
| Language | en_US | en_US |
| Publisher | PLOS (Public Library of Science) | en_US |
| dc.rights | cc-BY 4.0 | en_US |
| dc.source | PLOS One | en_US |
| dc.source.uri | http://journals.plos.org/plosone/ | en_US |
| Title | Distinct and Cooperative Activities of HESO1 and URT1 Nucleotidyl Transferases in MicroRNA Turnover in Arabidopsis | en_US |
| Type | Article | en_US |
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