A high-resolution radiation hybrid map of chicken chromosome 5 and comparison with human chromosomes

Abstract
The resolution of radiation hybrid (RH) maps is intermediate between that of the genetic and BAC (Bacterial Artificial Chromosome) contig maps. Moreover, once framework RH maps of a genome have been constructed, a quick location of markers by simple PCR on the RH panel is possible. The chicken ChickRH6 panel recently produced was used here to construct a high resolution RH map of chicken GGA5. To confirm the validity of the map and to provide valuable comparative mapping information, both markers from the genetic map and a high number of ESTs (Expressed Sequence Tags) were used. Finally, this RH map was used for testing the accuracy of the chicken genome assembly for chromosome 5.
Description
Final published version
Keywords
Citation
Pitel, F., Abasht, B., Morisson, M., Crooijmans, R. P. M. A., Vignoles, F., Leroux, S., . . . Vignal, A. (2004). A high-resolution radiation hybrid map of chicken chromosome 5 and comparison with human chromosomes. BMC Genomics, 5 doi:10.1186/1471-2164-5-66