Huang, Wen YanPan, Jeong HoonJeong, InhyeOh, Seong JuHyun, Yong GeonKim, Moon SeongHan, Bok KyungHong, JiyounKoo, Yong TaeLee, Ki WonJin, BoraNoh, DongjinKim, SanghoKim, Jae KyeomShin, Eui-CheolKim, Young Jun2023-02-162023-02-162022-12-12Huang, Wen Yan, Jeong Hoon Pan, Inhye Jeong, Seong Ju Oh, Yong Geon Hyun, Moon Seong Kim, Bok Kyung Han, et al. “Antifatigue and Anti-Inflammatory Effects of Cervus Elaphus L., Angelica Gigas Nakai, and Astragalus Membranaceus Bunge Complex Extracts in Physically Fatigued Mice.” Journal of Medicinal Food 25, no. 12 (December 12, 2022): 1126–32. https://doi.org/10.1089/jmf.2022.K.0103.1557-7600https://udspace.udel.edu/handle/19716/32308This is the accepted version of the following article: Huang, Wen Yan, Jeong Hoon Pan, Inhye Jeong, Seong Ju Oh, Yong Geon Hyun, Moon Seong Kim, Bok Kyung Han, et al. “Antifatigue and Anti-Inflammatory Effects of Cervus Elaphus L., Angelica Gigas Nakai, and Astragalus Membranaceus Bunge Complex Extracts in Physically Fatigued Mice.” Journal of Medicinal Food 25, no. 12 (December 12, 2022): 1126–32. https://doi.org/10.1089/jmf.2022.K.0103, which has now been formally published in final form at https://doi.org/10.1089/jmf.2022.K.0103. This original submission version of the article may be used for non-commercial purposes in accordance with the Mary Ann Liebert, Inc., publishers’ self-archiving terms and conditions.Fatigue is a common complaint among people under stress, causing an array of negative effects on physical function. In this study, we investigated the antifatigue and anti-inflammatory effects of Cervus elaphus L., Angelica gigas Nakai, and Astragalus membranaceus Bunge complex extracts (CAA) using a treadmill stress test in animal models. The mice were administered various doses of CAA (50–200 mg/kg bw per day) once daily for 21 days. After exhaustive treadmill exercise, the running time of CAA-treated mice increased 1.5 times; fatigue-related biochemical parameters, including lactate dehydrogenase (∼30%), creatine kinase (∼20%), and proinflammatory cytokines interleukin (IL)-1β (∼10%), and IL-6 (∼10%) in the serum and muscle tissue were downregulated compared with those in exercised control mice. This study provides strong evidence for the prevention of CAA-induced inflammatory incidences mediated by the blockade of nuclear factor-κB activation. Collectively, our results indicate that CAA can alleviate symptoms of fatigue in mice as an effective anti-inflammatory agent.en-USAngelica gigas Nakaiantifatigueanti-inflammatoryAstragalus membranaceusBungeCervus elaphus LArticle