Chang, Ming-Wen ChangYang, Jen-HaoTsitsipatis, DimitriosYang, XiaolingMartindale, Jennifer L.Munk, RachelPandey, Poonam R.Banskota, NiradRomero, BrigetteBatish, MonaPiao, YulanMazan-Mamczarz, KrystynaDe, SupriyoAbdelmohsen, KotbWilson, Gerald M.Gorospe, Myriam2023-02-202023-02-202022-12-19Ming-Wen Chang, Jen-Hao Yang, Dimitrios Tsitsipatis, Xiaoling Yang, Jennifer L Martindale, Rachel Munk, Poonam R Pandey, Nirad Banskota, Brigette Romero, Mona Batish, Yulan Piao, Krystyna Mazan-Mamczarz, Supriyo De, Kotb Abdelmohsen, Gerald M Wilson, Myriam Gorospe, Enhanced myogenesis through lncFAM-mediated recruitment of HNRNPL to the MYBPC2 promoter, Nucleic Acids Research, Volume 50, Issue 22, 19 December 2022, Pages 13026–13044, https://doi.org/10.1093/nar/gkac11741362-4962https://udspace.udel.edu/handle/19716/32328This article was originally publication in Nucleic Acids Research, published by Oxford University Press. The version of record is available at: https://doi.org/10.1093/nar/gkac1174The mammalian transcriptome comprises a vast family of long noncoding (lnc)RNAs implicated in physiologic processes such as myogenesis, through which muscle forms during embryonic development and regenerates in the adult. However, the specific molecular mechanisms by which lncRNAs regulate human myogenesis are poorly understood. Here, we identified a novel muscle-specific lncRNA, lncFAM71E1-2:2 (lncFAM), which increased robustly during early human myogenesis. Overexpression of lncFAM promoted differentiation of human myoblasts into myotubes, while silencing lncFAM suppressed this process. As lncFAM resides in the nucleus, chromatin isolation by RNA purification followed by mass spectrometry (ChIRP-MS) analysis was employed to identify the molecular mechanisms whereby it might promote myogenesis. Analysis of lncFAM-interacting proteins revealed that lncFAM recruited the RNA-binding protein HNRNPL to the promoter of MYBPC2, in turn increasing MYBPC2 mRNA transcription and enhancing production of the myogenic protein MYBPC2. These results highlight a mechanism whereby a novel ribonucleoprotein complex, lncFAM-HNRNPL, elevates MYBPC2 expression transcriptionally to promote myogenesis.en-USArticle