Merret, R´emyNagarajan, Vinay K.Carpentier, Marie-ChristinePark, SunheeFavory, Jean-JacquesDescombin, JuliePicart, ClaireCharng, Yee-yungGreen, Pamela J.Deragon, Jean-MarcBousquet-Antonelli, C´ ecile2015-12-232015-12-23Copyright2015-04-06Merret, Rémy, et al. "Heat-induced ribosome pausing triggers mRNA co-translational decay in Arabidopsis thaliana." Nucleic acids research (2015): gkv234.0305-1048 ; e- 1362-4962http://udspace.udel.edu/handle/19716/17345Publisher's PDFThe reprogramming of gene expression in heat stress is a key determinant to organism survival. Gene expression is downregulated through translation initiation inhibition and release of free mRNPs that are rapidly degraded or stored. In mammals, heat also triggers 5 -ribosome pausing preferentially on transcripts coding for HSC/HSP70 chaperone targets, but the impact of such phenomenon on mRNA fate remains unknown. Here, we provide evidence that, in Arabidopsis thaliana, heat provokes 5 -ribosome pausing leading to the XRN4-mediated 5 -directed decay of translating mRNAs. We also show that hindering HSC/HSP70 activity at 20◦C recapitulates heat effects by inducing ribosome pausing and co-translational mRNA turnover. Strikingly, co-translational decay targets encode proteins with high HSC/HSP70 binding scores and hydrophobic N-termini, two characteristics that were previously observed for transcripts most prone to pausing in animals. This work suggests for the first time that stress-induced variation of translation elongation rate is an evolutionarily conserved process leading to the polysomal degradation of thousands of ‘nonaberrant’ mRNAs.en-USCC-BY NC (http://creativecommons.org/licenses/by-nc/4.0/)Articledoi:10.1093/nar/gkv234