Biomimetic Proteoglycans Strengthen the Pericellular Matrix of Normal and Osteoarthritic Human Cartilage
| Author(s) | Kahle, Elizabeth R. | |
| Author(s) | Fallahi, Hooman | |
| Author(s) | Bergstrom, Annika R. | |
| Author(s) | Li, Anita | |
| Author(s) | Trouillot, Colette E. | |
| Author(s) | Mulcahey, Mary K. | |
| Author(s) | Lu, X. Lucas | |
| Author(s) | Han, Lin | |
| Author(s) | Marcolongo, Michele S. | |
| Date Accessioned | 2024-08-27T14:13:50Z | |
| Date Available | 2024-08-27T14:13:50Z | |
| Publication Date | 2024-08-12 | |
| Description | This article was originally published in ACS Biomaterials Science & Engineering. The version of record is available at: https://doi.org/10.1021/acsbiomaterials.4c00813. © 2024 The Authors. Published by American Chemical Society | |
| Abstract | In osteoarthritis (OA), degradation of cartilage pericellular matrix (PCM), the proteoglycan-rich immediate cell microniche, is a leading event of disease initiation. This study demonstrated that biomimetic proteoglycans (BPGs) can diffuse into human cartilage from both normal and osteoarthritic donors and are preferentially localized within the PCM. Applying immunofluorescence (IF)-guided AFM nanomechanical mapping, we show that this localization of BPGs increases the PCM micromodulus of both normal and OA specimens. These results illustrate the capability of BPGs to integrate with degenerative tissues and support the translational potential of BPGs for treating human OA and other diseases associated with proteoglycan degradation. | |
| Sponsor | This work was financially supported by the National Science Foundation (NSF) grant CMMI-1826202 (to M.S.M. and L.H.), National Institutes of Health (NIH) grant R01AR080139 (to M.S.M.), R01AR074490 (to L.H.) and R01AR074472 (to X.L.L.), as well as NIH grant P30AR069619 to the Penn Center for Musculoskeletal Disorders. We thank the Singh Center for Nanotechnology at the University of Pennsylvania for the use of TIRF MFP-3D, which is part of the National Nanotechnology Coordinated Infrastructure Program supported by the NSF grant NNCI-1542153. All imaging was performed at Drexel University’s Cell Imaging Center. | |
| Citation | Kahle, Elizabeth R., Hooman Fallahi, Annika R. Bergstrom, Anita Li, Colette E. Trouillot, Mary K. Mulcahey, X. Lucas Lu, Lin Han, and Michele S. Marcolongo. “Biomimetic Proteoglycans Strengthen the Pericellular Matrix of Normal and Osteoarthritic Human Cartilage.” ACS Biomaterials Science & Engineering, August 12, 2024. https://doi.org/10.1021/acsbiomaterials.4c00813. | |
| ISSN | 2373-9878 | |
| URL | https://udspace.udel.edu/handle/19716/34894 | |
| Language | en_US | |
| Publisher | ACS Biomaterials Science & Engineering | |
| dc.rights | Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| Keywords | biomimetic proteoglycan | |
| Keywords | pericellular matrix | |
| Keywords | osteoarthritis | |
| Keywords | IF-guided AFM | |
| Keywords | molecular engineering | |
| Title | Biomimetic Proteoglycans Strengthen the Pericellular Matrix of Normal and Osteoarthritic Human Cartilage | |
| Type | Article |
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