Harnessing multifunctional collagen mimetic peptides to create bioinspired stimuli responsive hydrogels for controlled cell culture

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Author(s)Ford, Eden M.
Author(s)Hilderbrand, Amber M.
Author(s)Kloxin, April M.
Date Accessioned2024-11-06T15:21:29Z
Date Available2024-11-06T15:21:29Z
Publication Date2024-08-12
DescriptionThis article was originally published in Journal of Materials Chemistry B. The version of record is available at: https://doi.org/10.1039/D4TB00562G. This journal is © The Royal Society of Chemistry 2024. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence (http://creativecommons.org/licenses/by-nc/3.0/).
AbstractThe demand for synthetic soft materials with bioinspired structures continues to grow. Material applications range from in vitro and in vivo tissue mimics to therapeutic delivery systems, where well-defined synthetic building blocks offer precise and reproducible property control. This work examines a synthetic assembling peptide, specifically a multifunctional collagen mimetic peptide (mfCMP) either alone or with reactive macromers, for the creation of responsive hydrogels that capture aspects of soft collagen-rich tissues. We first explored how buffer choice impacts mfCMP hierarchical assembly, in particular, peptide melting temperature, fibril morphology, and ability to form physical hydrogels. Assembly in physiologically relevant buffer resulted in collagen-like fibrillar structures and physically assembled hydrogels with shear-thinning (as indicated through strain-yielding) and self-healing properties. Further, we aimed to create fully synthetic, composite peptide-polymer hydrogels with dynamic responses to various stimuli, inspired by the extracellular matrix (ECM). Specifically, we established mfCMP–poly(ethylene glycol) (PEG) hydrogel compositions that demonstrate increasing non-linear viscoelasticity in response to applied strain as the amount of assembled mfCMP content increases. Furthermore, the thermal responsiveness of mfCMP physical crosslinks was harnessed to manipulate the composite hydrogel mechanical properties in response to changes in temperature. Finally, cells relevant in wound healing, human lung fibroblasts, were encapsulated within these peptide–polymer hydrogels to explore the impact of increased mfCMP, and the resulting changes in viscoelasticity, on cell response. This work establishes mfCMP building blocks as versatile tools for creating hybrid and adaptable systems with applications ranging from injectable shear-thinning materials to responsive interfaces and synthetic ECMs for tissue engineering.
SponsorWe acknowledge financial support from the New Innovator Award funded by the National Institutes of Health (NIH) (DP2-HL152424), Pew Charitable Trusts (26178), and the University of Delaware Center for Hybrid, Active, and Responsive Materials (UD CHARM) Materials Research Science and Engineering Center (MRSEC) program supported by the National Science Foundation (NSF) (DMR-2011824). Support for instrumentation at the University of Delaware was provided in part by the Delaware Centers of Biomedical Research Excellence (COBRE) programs supported by grants funded by an Institutional Development Award from the National Institute of General Medical Sciences (NIGMS) of the NIH (P20GM104316, 5 P30 GM110758-02) and the UD CHARM MRSEC program supported by the NSF (DMR-2011824). This publication was also made possible by UD Core facilities including the Keck Center for Advanced Microscopy and Microanalysis and the University of Delaware NMR and Mass Spectrometry Core facilities. The authors would like to acknowledge the Millicent Sullivan group for their use of equipment and Christopher Kloxin for discussions regarding hydrogel properties.
CitationFord, Eden M., Amber M. Hilderbrand, and April M. Kloxin. “Harnessing Multifunctional Collagen Mimetic Peptides to Create Bioinspired Stimuli Responsive Hydrogels for Controlled Cell Culture.” Journal of Materials Chemistry B 12, no. 38 (2024): 9600–9621. https://doi.org/10.1039/D4TB00562G.
ISSN2050-7518
URLhttps://udspace.udel.edu/handle/19716/35493
Languageen_US
PublisherJournal of Materials Chemistry B
dc.rightsAttribution-NonCommercial 3.0 Unported Licenceen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/
TitleHarnessing multifunctional collagen mimetic peptides to create bioinspired stimuli responsive hydrogels for controlled cell culture
TypeArticle
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