Zonal patterning of extracellular matrix and stromal cell populations along a perfusable cellular microchannel

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Author(s)Chernokal, Brea
Author(s)Ferrick, Bryan J.
Author(s)Gleghorn, Jason P.
Date Accessioned2024-12-12T19:19:11Z
Date Available2024-12-12T19:19:11Z
Publication Date2024-10-21
DescriptionThis article was originally published in Lab on a Chip. The version of record is available at: https://doi.org/10.1039/D4LC00579A. This journal is © The Royal Society of Chemistry 2024. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence (http://creativecommons.org/licenses/by-nc/3.0/).
AbstractThe spatial organization of biophysical and biochemical cues in the extracellular matrix (ECM) in concert with reciprocal cell–cell signaling is vital to tissue patterning during development. However, elucidating the role an individual microenvironmental factor plays using existing in vivo models is difficult due to their inherent complexity. In this work, we have developed a microphysiological system to spatially pattern the biochemical, biophysical, and stromal cell composition of the ECM along an epithelialized 3D microchannel. This technique is adaptable to multiple hydrogel compositions and scalable to the number of zones patterned. We confirmed that the methodology to create distinct zones resulted in a continuous, annealed hydrogel with regional interfaces that did not hinder the transport of soluble molecules. Further, the interface between hydrogel regions did not disrupt microchannel structure, epithelial lumen formation, or media perfusion through an acellular or cellularized microchannel. Finally, we demonstrated spatially patterned tubulogenic sprouting of a continuous epithelial tube into the surrounding hydrogel confined to local regions with stromal cell populations, illustrating spatial control of cell–cell interactions and signaling gradients. This easy-to-use system has wide utility for modeling three-dimensional epithelial and endothelial tissue interactions with heterogeneous hydrogel compositions and/or stromal cell populations to investigate their mechanistic roles during development, homeostasis, or disease.
SponsorThe authors thank Katherine M. Nelson, Ph.D., for reviewing and commenting on the manuscript. This work was supported in part by grants from the National Institutes of Health: R01DE029655 and R01HL145147, and the National Science Foundation GRFP (1940700) (B. C.). Microscopy equipment was acquired with a shared instrumentation grant (S10 OD030321) and access was supported by NIH-NIGMS (P20 GM103446, P20 GM139760) and the State of Delaware.
CitationChernokal, Brea, Bryan J. Ferrick, and Jason P. Gleghorn. “Zonal Patterning of Extracellular Matrix and Stromal Cell Populations along a Perfusable Cellular Microchannel.” Lab on a Chip 24, no. 23 (2024): 5238–50. https://doi.org/10.1039/D4LC00579A.
ISSN1473-0189
URLhttps://udspace.udel.edu/handle/19716/35654
Languageen_US
PublisherLab on a Chip
dc.rightsAttribution-NonCommercial 3.0 Unporteden
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/
TitleZonal patterning of extracellular matrix and stromal cell populations along a perfusable cellular microchannel
TypeArticle
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