Kinetics and Retention of Polystyrenesulfonate for Proteoglycan Replacement in Cartilage
Date
2024-08-14
Journal Title
Journal ISSN
Volume Title
Publisher
Biomacromolecules
Abstract
Tissue hydration provides articular cartilage with dynamic viscoelastic properties. Early stage osteoarthritis (OA) is marked by loss of proteoglycans and glycosaminoglycans (GAG), lowering fixed charge density, and impairing tissue osmotic function. The most common GAG replacement, chondroitin sulfate (CS), has failed to show effectiveness. Here, we investigated a synthetic polyelectrolyte, poly(styrenesulfonate) (PSS), both as a model compound to investigate polyelectrolyte transport in cartilage, and as a potential candidate to restore bulk fixed charge density in cartilage with GAG loss. Through bovine explants and histology, we determined zonal-based effective diffusion coefficients for three different molecular weights of PSS. Compared to CS, PSS was retained longer in GAG-depleted cartilage in static and compression-based desorption experiments. We explained enhanced solute performance of PSS by its more compact morphology and higher charge density by small-angle X-ray scattering. This study may improve design of GAG mimetic molecules for repairing osmotic function in OA cartilage.
Description
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biomacromolecules, copyright © 2024 The Authors. Published by American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://www.doi.org/10.1021/acs.biomac.4c00479. This article will be embargoed until 08/14/2025.
Keywords
GAG replacement, proteoglycan replacement, osteoarthritis, fixed charge density, synthetic polyelectrolytes
Citation
Sundar, Shalini, Allison Koopman, Thomas J. Manzoni, Weiran Xie, Qurat-Ul-Ain Bhatti, Chun-Yuan Lo, Vidhika S. Damani, et al. “Kinetics and Retention of Polystyrenesulfonate for Proteoglycan Replacement in Cartilage.” Biomacromolecules 25, no. 9 (September 9, 2024): 5819–33. https://doi.org/10.1021/acs.biomac.4c00479.