Time resolved and steady state experiments with PRODAN and laurdan solutions
Date
2019
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
University of Delaware
Abstract
2-propionyl-6-dimethylamino naphthalene (PRODAN) is used in biomedical
research due to the sensitivity of its emission spectrum to changes in the environment.
Typically, the fluorescence intensity at two wavelengths is analyzed to draw
conclusions about water content, for example, of the PRODAN’s microenvironment.
However, both polarity and hydrogen bonding ability of the environment play roles in
PRODAN’s emission behavior. PRODAN was studied in a variety of solvents and
mixtures, using steady state spectroscopy and time resolved techniques to bring a new
perspective to the discussion of the nature of the excited states of PRODAN and the
factors which give rise to them.