Models of the tear film interaction with the ocular surface

Date
2013-05
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University of Delaware
Abstract
Mathematical models that connect the tear lm with the underlying epithelia of the cornea and conjunctiva were developed. The tear lm is assumed to be a spatially uniform evaporating aqueous layer with a concentration variable representing the osmolarity in the tear lm. In the rst model, the underlying epithelia are approximated as in Levin and Verkmann [6], with a few layers of cells of varying thickness that are linked via osmosis between each of the cells and with the tear lm. Loss of water due to evaporation from the tear lm causes increased osmolarity there, leading to osmotic ow from the epithelium to the tear lm, and between the epithelial cells. The parameters are set by comparison with thinning rate measurements from human subjects in vivo. In those experiments, 5% uores- cein solution is instilled in the tear lm, and the thickness of the tear lm is deduced from subsequent intensity measurements in the concentrated ourescein regime. These measure- ments were used to calibrate the model parameters for osmosis. A second model is proposed that includes the molecules involved in metabolism in the epithelial cell of the cornea. This model is based on the Leung et al model [3]. The proposed model includes di erential and algebraic equations to represent the concentrations of the metabolites in the cells and their e ects over time. Both models are used to predict tear lm thickness and osmolarity, as well as epithelial cell layer thicknesses and osmolarities. The rst model predicts thickness and osmolarity changes for single interblinks and for extended periods of many blink cycles with constant interblink duration. Cumulative e ects from multiple blink cycles are seen that are signi cantly more than for single interblinks. We interpret the results in light of biochemical response and the rate of progression of apoptosis in squamous cells.
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