Biophysical modelling of intrinsic cardiac nervous system neuronal electrophysiology based on single-cell transcriptomics
Date
2025-03-12
Journal Title
Journal ISSN
Volume Title
Publisher
The Journal of Physiology
Abstract
The intrinsic cardiac nervous system (ICNS), termed as the heart's ‘little brain’, is the final point of neural regulation of cardiac function. Studying the dynamic behaviour of these ICNS neurons via multiscale neuronal computer models has been limited by the sparsity of electrophysiological data. We developed and analysed a computational library of neuronal electrophysiological models based on single neuron transcriptomic data obtained from ICNS neurons. Each neuronal genotype was characterized by a unique combination of ion channels identified from the transcriptomic data, using a cycle threshold cutoff that ensured the electrical excitability of the neuronal models. The parameters of the ion channel models were grounded based on passive properties (resting membrane potential, input impedance and rheobase) to avoid biasing the dynamic behaviour of the model. Consistent with experimental observations, the emergent model dynamics showed phasic activity in response to the current clamp stimulus in a majority of neuronal genotypes (61%). Additionally, 24% of the ICNS neurons showed a tonic response, 11% were phasic-to-tonic with increasing current stimulation and 3% showed tonic-to-phasic behaviour. The computational approach and the library of models bridge the gap between widely available molecular-level gene expression and sparse cellular-level electrophysiology for studying the functional role of the ICNS in cardiac regulation and pathology.
Description
This article was originally published in The Journal of Physiology. The version of record is available at: https://doi.org/10.1113/JP287595.
© 2025 The Author(s). The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.
This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords
autonomic regulation, Hodgkin–Huxley model, intrinsic cardiac nervous system, ion channels, systems biology, vagus nerve
Citation
Gupta, S., Gee, M.M., Newton, A.J.H., Kuttippurathu, L., Moss, A., Tompkins, J.D., Schwaber, J.S., Vadigepalli, R. and Lytton, W.W. (2025), Biophysical modelling of intrinsic cardiac nervous system neuronal electrophysiology based on single-cell transcriptomics. J Physiol, 603: 2119-2138. https://doi.org/10.1113/JP287595