Standard purification methods are not sufficient to remove micellular lipophilic dye from polymer nanoparticle solution
| Author(s) | Sterin, Eric H. | |
| Author(s) | Weinstein, Laura A. | |
| Author(s) | Chowdhury, Chitran Roy | |
| Author(s) | Guzzetti, Emma C. | |
| Author(s) | Day, Emily S. | |
| Date Accessioned | 2025-03-24T18:17:58Z | |
| Date Available | 2025-03-24T18:17:58Z | |
| Publication Date | 2025-03-06 | |
| Description | This article was originally published in RSC Pharmaceutics. The version of record is available at: https://doi.org/10.1039/D5PM00013K. © 2025 The Author(s). Published by the Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence (http://creativecommons.org/licenses/by-nc/3.0/). | |
| Abstract | Tracking nanoparticles’ location is imperative for understanding cellular interactions, pharmacokinetics, and biodistribution. DiD is a lipophilic dye commonly used to label nanoparticles for trafficking studies. Herein, we show that DiD micelles form in polymer NP solutions during synthesis and can lead to false positive results in downstream assays. Potential methods to remove these micelles are also described. | |
| Sponsor | The authors thank Dr John Sperduto for his input and expertise as well as the Delaware Biotechnology Institute for their support. This work was supported by the National Institutes of Health under award numbers R35GM149292 and R35GM119659 and by the University of Delaware Summer Scholars Program. E. H. S. acknowledges support from the National Science Foundation Graduate Research Fellowship Program under award number 1940700. | |
| Citation | Sterin, Eric H., Laura A. Weinstein, Chitran Roy Chowdhury, Emma C. Guzzetti, and Emily S. Day. “Standard Purification Methods Are Not Sufficient to Remove Micellular Lipophilic Dye from Polymer Nanoparticle Solution.” RSC Pharm., 2025. https://doi.org/10.1039/D5PM00013K. | |
| ISSN | 2976-8713 | |
| URL | https://udspace.udel.edu/handle/19716/35970 | |
| Language | en_US | |
| Publisher | RSC Pharmaceutics | |
| dc.rights | Attribution-NonCommercial 3.0 Unported | en |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/ | |
| Title | Standard purification methods are not sufficient to remove micellular lipophilic dye from polymer nanoparticle solution | |
| Type | Article |
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