Standard purification methods are not sufficient to remove micellular lipophilic dye from polymer nanoparticle solution

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dc.contributor.authorSterin, Eric H.
dc.contributor.authorWeinstein, Laura A.
dc.contributor.authorChowdhury, Chitran Roy
dc.contributor.authorGuzzetti, Emma C.
dc.contributor.authorDay, Emily S.
dc.date.accessioned2025-03-24T18:17:58Z
dc.date.available2025-03-24T18:17:58Z
dc.date.issued2025-03-06
dc.descriptionThis article was originally published in RSC Pharmaceutics. The version of record is available at: https://doi.org/10.1039/D5PM00013K. © 2025 The Author(s). Published by the Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence (http://creativecommons.org/licenses/by-nc/3.0/).
dc.description.abstractTracking nanoparticles’ location is imperative for understanding cellular interactions, pharmacokinetics, and biodistribution. DiD is a lipophilic dye commonly used to label nanoparticles for trafficking studies. Herein, we show that DiD micelles form in polymer NP solutions during synthesis and can lead to false positive results in downstream assays. Potential methods to remove these micelles are also described.
dc.description.sponsorshipThe authors thank Dr John Sperduto for his input and expertise as well as the Delaware Biotechnology Institute for their support. This work was supported by the National Institutes of Health under award numbers R35GM149292 and R35GM119659 and by the University of Delaware Summer Scholars Program. E. H. S. acknowledges support from the National Science Foundation Graduate Research Fellowship Program under award number 1940700.
dc.identifier.citationSterin, Eric H., Laura A. Weinstein, Chitran Roy Chowdhury, Emma C. Guzzetti, and Emily S. Day. “Standard Purification Methods Are Not Sufficient to Remove Micellular Lipophilic Dye from Polymer Nanoparticle Solution.” RSC Pharm., 2025. https://doi.org/10.1039/D5PM00013K.
dc.identifier.issn2976-8713
dc.identifier.urihttps://udspace.udel.edu/handle/19716/35970
dc.language.isoen_US
dc.publisherRSC Pharmaceutics
dc.rightsAttribution-NonCommercial 3.0 Unporteden
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/
dc.titleStandard purification methods are not sufficient to remove micellular lipophilic dye from polymer nanoparticle solution
dc.typeArticle

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