Prohibitin involvement in the generation of mitochondrial superoxide at complex I in human sperm

Author(s)Chai, Ran-Ran
Author(s)Chen, Guo-Wu
Author(s)Shi, Hui-Juan
Author(s)Martin-DeLeon, Patricia A
Author(s)Chen, Hong
Author(s)Wai-Sum, O
Ordered AuthorRan-Ran Chaien_US
Ordered AuthorGuo-Wu Chenen_US
Ordered AuthorHui-Juan Shien_US
Ordered AuthorWai-Sum Oen_US
Ordered AuthorPatricia A. Martin-DeLeonen_US
Ordered AuthorHong Chenen_US
UD AuthorMartin-DeLeon, Patricia Aen_US
Date Accessioned2017-03-10T15:39:14Z
Date Available2017-03-10T15:39:14Z
Copyright Date2016 The Authorsen_US
Publication Date2016-08-25
DescriptionPublisher's PDFen_US
AbstractProhibitin (PHB), a major mitochondrial membrane protein, has been shown earlier in our laboratoryto regulate sperm motility via an alteration in mitochondrial membrane potential (MMP) in infertile men with poor sperm quality. To test if PHB expression is associated with sperm mitochondrial superoxide (mROS) levels, here we examined sperm mROS levels, high MMP and lipid peroxidation in infertile men with poor sperm motility (asthenospermia, A) and/or low sperm concentrations (oligoasthenospermia, OA). The diaphorase-type activity of sperm mitochondrial complex I (MCI) and PHB expression were also determined. We demonstrate that mROS and lipid peroxidation levels are significantly higher in sperm from A and OA subjects than in normospermic subjects, whereas high MMP and PHB expression are significantly lower. A positive correlation between mROS and lipid peroxidation and a negative correlation of mROS with PHB expression, high MMP, and sperm motility were found in these subjects. The finding of similar diaphorase-type activity levels of sperm MCI in the three groups studied suggests that the catalytic subunits of MCI in the matrix arm may produce mROS on its own. There may be a dysfunction of electron transport at MCI associated with decreased expression of PHB in sperm with poor quality. We conclude that mROS level is increased and associated with decreased PHB expression, and it may regulate sperm motility via increases in low MMP and lipid peroxidation. This is the first report on the involvement of PHB in human sperm motility loss associated with increased generation of mROS at MCI.en_US
DepartmentDepartment of Biological Sciences, University of Delaware, Newark, DE, USAen_US
CitationChai, R., Chen, G., Shi, H., O, W., Martin‐DeLeon, P. A., & Chen, H. (2017). Prohibitin involvement in the generation of mitochondrial superoxide at complex I in human sperm. Journal of Cellular and Molecular Medicine, 21(1), 121–129. http://doi.org/10.1111/jcmm.12945en_US
DOI10.1111/jcmm.12945en_US
ISSN1582-4934en_US
URLhttp://udspace.udel.edu/handle/19716/21141
LanguageEnglishen_US
PublisherWiley-Blackwellen_US
dc.rightsCC BY 4.0en_US
dc.sourceJournal of Cellular and Molecular Medicineen_US
dc.source.urihttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1582-4934en_US
TitleProhibitin involvement in the generation of mitochondrial superoxide at complex I in human spermen_US
TypeArticleen_US
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