Postnatal binge-like alcohol exposure reduces spine density without affecting dendritic morphology in rat medial prefrontal cortex
| Author(s) | Whitcher, Lee T. | |
| Date Accessioned | 2020-10-07T12:49:12Z | |
| Date Available | 2020-10-07T12:49:12Z | |
| Publication Date | 2007 | |
| Abstract | Amongst the deficits associated with fetal alcohol syndrome (FAS), cognitive impairments are among the most debilitating and long-lasting. These impairments, including deficits in goal-directed behavior, attention, temporal planning, and other executive functions, could result from damage to the prefrontal cortex (PFC), an area that has not been studied sufficiently in the context of FAS. Neuronal connectivity in this area, as measured by distribution of dendritic spines and the complexity of dendritic tree structure, can be influenced by exogenous variables other than alcohol, and the neuronal connectivity in other brain regions can be affected by alcohol exposure. The goal of this study was to determine whether binge-like alcohol exposure on postnatal days (PD) 4-9 affects dendritic spine density and other dendritic tree parameters in mPFC that could possibly underlie functional damage. Rats were intubated with alcohol (5.25g/kg/day; AE - alcohol exposed), sham intubated (SI), or remained with the mother (SC - suckle control) on PD4-9. Animals were sacrificed between PD 26-30 and brains were processed for Golgi-Cox staining. Apical dendrite complexity and spine density were evaluated for layer III neurons in the mPFC using NeuroLucida software (MicroBrightField, Inc.). Spine density was significantly decreased in AE animals relative to SI and SC controls, but no differences in dendritic complexity were found across experimental groups. Our findings demonstrate that neonatal alcohol exposure has a persistent effect on the spine density in mPFC that can explain functional deficits in this cortical area. | en_US |
| Advisor | Klintsova, Anna | |
| Degree | M.A. | |
| Department | University of Delaware, Department of Psychology | |
| DOI | https://doi.org/10.58088/g0g1-xq80 | |
| Unique Identifier | 225007859 | |
| URL | https://udspace.udel.edu/handle/19716/27808 | |
| Publisher | University of Delaware | en_US |
| URI | https://login.udel.idm.oclc.org/login?url=https://www.proquest.com/docview/304859350?accountid=10457 | |
| dc.subject.lcsh | Fetal alcohol spectrum disorders | |
| dc.subject.lcsh | Rats -- Physiology | |
| dc.subject.lcsh | Prefrontal cortex | |
| dc.subject.lcsh | Alcohol -- Physiological effect | |
| dc.subject.lcsh | Spine -- Abnormalities | |
| Title | Postnatal binge-like alcohol exposure reduces spine density without affecting dendritic morphology in rat medial prefrontal cortex | en_US |
| Type | Thesis | en_US |