Glucose detection with surface plasmon resonance spectroscopy and molecularly imprinted hydrogel coated sensors
Date
2011
Authors
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Publisher
University of Delaware
Abstract
Molecularly imprinted hydrogel membranes were developed and evaluated for
detection of small analytes via surface plasmon resonance spectroscopy. Imprinting of
glucose phosphate barium salt into a poly(allylamine hydrochloride) network covalently
bound to gold surfaces yielded a selective sensor for glucose. Optimization of relative
amounts of chemicals used for preparation of the hydrogel was performed to obtain
highest sensitivity. Addition of gold nanoparticles into the hydrogel matrix significantly
amplified its response and sensitivity due to the impact of gold nanoparticles on the
refractive index of sensing layer. The detection limit of glucose in deionized water was
calculated to be 0.02 mg/mL, well within the working concentration range suitable for
glucose monitoring in diabetic individuals at physiological levels. Evaluation of its
selectivity showed that the sensor displayed preferential recognition to glucose compared
to structurally related sugars in addition to being unaffected by phosphate as well as
compounds containing amine groups, such as creatinine. The developed sensor was
finally exposed to human urine spiked with glucose illustrating its ability to selectively
re-bind the analyte in complex matrices.